Coupon
Crispr-Cas : A Laboratory Manual
by Jennifer Doudna and Prashant Mali


Overview - The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.  Read more...

 
In Stock.

This item is Non-Returnable.
FREE Shipping for Club Members
 
> Check In-Store Availability

In-Store pricing may vary

 
 
New & Used Marketplace 14 copies from $94.02
 
 
 
 

More About Crispr-Cas by Jennifer Doudna; Prashant Mali
 
 
 
Overview
The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.

Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.

Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.


This item is Non-Returnable.

 
Details
  • ISBN-13: 9781621821311
  • ISBN-10: 1621821315
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publish Date: March 2016
  • Page Count: 192
  • Dimensions: 10.8 x 8.3 x 0.5 inches
  • Shipping Weight: 1.3 pounds


Related Categories

Books > Science > Life Sciences - Biochemistry
Books > Science > Life Sciences - Molecular Biology
Books > Medical > Microbiology

 
BAM Customer Reviews