{ "item_title" : "Data-Driven Modeling of Molecular Interactions at the Trans-Golgi Network of Mammalian Cells", "item_author" : [" Patrick Weber "], "item_description" : "In this thesis simulation models have been developed and validated to investigate the protein-lipid interaction mechanisms at the trans-Golgi network (TGN) of mammalian cells. In two systems biological studies, ordinary differential equation models were used to examine the interactions between the lipids involved in the sphingomyelin synthase 1 reaction at the TGN and the proteins involved in the regulation of non-vesicular Endoplasmic reticulum to TGN ceramide transfer. These systems include the lipids ceramide, phosphatidyl choline, diacylglycerol and sphingomyelin and the proteins protein kinase D (PKD), phosphatidyl inositol-4-kinase III ss and ceramide transfer protein (CERT), respectively. The final results comprise a quantitative model of this network, and a comparison of competing hypotheses regarding the mechanism of ceramide transfer. Major biological findings are that PKD and CERT work together in a cooperative manner to perform ceramide transfer by forming a positive feedback regulation. Important methodological side results of this thesis are a novel absolute quantification scheme for proteins via Western Blot data and a Bayesian experiment design method.", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/3/83/254/023/3832540237_b.jpg", "price_data" : { "retail_price" : "60.00", "online_price" : "60.00", "our_price" : "60.00", "club_price" : "60.00", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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