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Assay Development|Ge Wu

Assay Development : Fundamentals and Practices

by Ge Wu
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Overview

Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:

  • The basics of assay development, including foundational concepts and applications

  • Commonly used instrumental methods for both biochemical assays and cell-based assays

  • Assay strategies for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.

This item is Non-Returnable

Details

  • ISBN-13: 9780470191156
  • ISBN-10: 0470191155
  • Publisher: Wiley
  • Publish Date: April 2010
  • Dimensions: 9.3 x 6.1 x 1.1 inches
  • Shipping Weight: 1.65 pounds
  • Page Count: 448

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