Overview
Chapter 1 - Introduction Overview of Single Molecule Force Spectroscopy Techniques Experiments Using Single molecule AFM Limitations of AFM-based single molecule force spectroscopy Summary Chapter 2- Correlation Force Spectroscopy Correlation Force Spectroscopy: Rationale Correlation Force Spectroscopy: Development Laterally Offset Configuration Vertically Offset Configuration Analysis of Correlations between Two Cantilevers Validation of Fluctuation-Dissipation Theorem for One Cantilever Analysis of Thermal Fluctuations to obtain Correlations Chapter 3- Dynamics of Single Molecules Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain Models Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model Internal Friction Rouse with Internal Friction Model of Linear Viscoelasticity of a Semiflexible Chain Summary Chapter 4- Microrheology with Correlation Force Spectroscopy Existing Techniques of Rheometry Experimental Methods Comparison to Finite Element Analysis Comparison to Simple Harmonic Oscillator Model Summary Chapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study Materials and Methods Analysis Results Discussion Summary Chapter 6- Correlation Force Spectroscopy for Single Molecule Measurements Effect of the Distance between Cantilever Tips Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy Summary Chapter 7- Single Molecule Force Spectroscopy of Dextran Materials and Methods Results Stretching a Dextran Molecule Static Force-Elongation Mode Dynamic Correlated Fluctuations Mode Discussion Summary Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNA Rationale Introduction Materials and Methods Modal Analysis Results Discussion Summary Chapter 9- Summary An Overview of Chapters Future Work Appendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force Spectroscopy Appendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered Molecule References
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Details
- ISBN-13: 9783319386409
- ISBN-10: 3319386409
- Publisher: Springer
- Publish Date: October 2016
- Dimensions: 9.21 x 6.14 x 0.32 inches
- Shipping Weight: 0.48 pounds
- Page Count: 117
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