{ "item_title" : "Cytochrome P450 Protocols", "item_author" : [" Ian R. Phillips", "Elizabeth A. Shephard", "Paul R. Ortiz De Montellano "], "item_description" : "1. Bioluminescent Assays for Cytochrome P450 Enzymes Douglas S. Auld, Henrike Veith, and James J. Cali 2. In Vitro Drug-Drug Interaction AssayMichael Zientek and Kuresh Youdim 3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel 4. The Synthesis, Characterization and Application of 13 C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem 5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based InactivatorsCesar Kenaan, Haoming Zhang, and Paul F. Hollenberg 6. Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches Qian Cheng and F. Peter. Guengerich 7. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, and Paul R. Ortiz de Montellano 8. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme ProteinsMichael B. Winter,Joshua J. Woodward,and Michael A. Marletta 9. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group Wesley E. Straub, Clinton R. Nishida, and Paul R. Ortiz de Montellano 10. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 EnzymesA. Luthra, M. Gregory, Y.V. Grinkova, I.G. Denisov, and S.G. Sligar 11. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles Linlin Zhao,Besnik Bajrami,and James F. Rusling 12. Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells M. Teresa Donato and M. JosG mez-Lech n 13. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme Kersten S. Rabe and Christof M. Niemeyer 14. High-Throughput Fluorescence Assay of Cytochrome P450 3A4Qian Cheng and F. Peter. Guengerich 15. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler 16. DNA Shuffling of Cytochrome P450 Enzymes James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam 17. Measurement of P450 Difference Spectra Using Intact Cells Wayne A. Johnston and Elizabeth M.J. Gillam 18. DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production Nedeljka N. Rosic 19. P450 oxidoreductase - Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein Vishal Agrawal and Walter L. Miller 20. LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450 Federico Sabbadin, Gideon G. Grogan and Neil C. Bruce 21. Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database Sarah C. Sim and Magnus Ingelman-Sundberg 22. Simultaneous in vivo Phenotyping of CYP Enzymes Sussan Ghassabian and Michael Murray 23. Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting Matthew Ha", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/1/62/703/320/1627033203_b.jpg", "price_data" : { "retail_price" : "169.99", "online_price" : "169.99", "our_price" : "169.99", "club_price" : "169.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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