{ "item_title" : "Enzyme Engineering", "item_author" : [" James C. Samuelson "], "item_description" : "1. A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivoLinda Foit and James Bardwell2. Determining Enzyme Kinetics via Isothermal Titration CalorimetryNeil A. Demarse, Marie C. Killian, Lee D. Hansen, and Colette F. Quinn3. GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in BacteriaOlga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou, and Everett Stone4. Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage PropertiesSarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg, and Jordan Jarjour5. TAL Effector Nuclease (TALEN) EngineeringTing Li and Bing Yang6. In vitro Evolution of EnzymesMisha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone, and Burckhard Seelig7. Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivoAmrita Signh-Blom, Randall A. Hughes, and Andrew. D. Ellington8. Reconstructing Evolutionary Adaptive Paths for Protein EngineeringMegan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher9. Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in BacteriaBryan M. Swingle10. FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme VariantsEric R. Geertsma11. Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme ComplexesHidehiko Hirakawa and Teruyuki Nagamune12. Gene Synthesis by Assembly of Deoxyuridine Containing OligonucleotidesRomualdas Vaisvila and Jurate Bitinaite13. Protein Engineering: Single or Multiple Site-Directed MutagenesisPei-Chung Hsieh and Romualdas Vaisvila14. Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative RecombinationNili Ostrov, Laura M. Wingler, and Virginia Cornish15. Promiscuity-Based Enzyme Selection for Rational Directed Evolution ExperimentsSandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. dandekar, Swapan K. Bhattacharjee, and Basuthkar J. Rao16. Rational Protein Sequence Diversification by Multi-Codon Scanning MutagenesisJia Liu and T. Ashton Cropp17. Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a ReporterJian-Wei Liu and David Ollis18. In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre PlatesBradley J. Stevenson, Sylvia H.C. Yip, and David L. Ollis", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/1/62/703/292/1627032924_b.jpg", "price_data" : { "retail_price" : "169.99", "online_price" : "169.99", "our_price" : "169.99", "club_price" : "169.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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