{ "item_title" : "Essential Genes and Genomes", "item_author" : [" Ren Zhang "], "item_description" : "1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell LinesKimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential GenesBert Kwanten, Jasper Neggers, and Dirk Daelemans4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicansLauren Wensing and Rebecca S. Shapiro5. Identification of Essential Genes Using Sequential CRISPR and siRNA ScreensLuke DeHart, Oliver P. Yockey, and Jesse Bakke6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell LinesKohei Nishimura and Tatsuo Fukagawa7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference ScreenXihao Liao, Xin-Hui Xing, and Chong Zhang8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in CyanobacteriaJu-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human SerumMiguel Carda-Di guez and Carmen Amaro10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosaBradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed SequencingAlex J. McCarthy and Peter W. Taylor12. Using Genome Scale Mutant Libraries to Identify Essential GenesKevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon SequencingAlejandro R. Walker and Robert C. Shields14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle SystemsGayatri Sharma and Patrick D. Curtis15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus ", "item_img_path" : "https://covers4.booksamillion.com/covers/bam/1/07/161/719/1071617192_b.jpg", "price_data" : { "retail_price" : "249.99", "online_price" : "249.99", "our_price" : "249.99", "club_price" : "249.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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