{ "item_title" : "Gap Junction Protocols", "item_author" : [" Mathieu Vinken", "Scott R. Johnstone "], "item_description" : "1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain ReactionMicha l Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken2. DNA Methylation Analysis of Human Tissue-Specific Connexin GenesXiaoming Liu and Canxia Xu3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot AnalysisJoost Willebrords, Micha l Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken4. Immunohisto- and Cytochemistry Analysis of ConnexinsBruno Cogliati, Micha l Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle CellsMiranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and LaborMark Kibschull, Stephen J. Lye, and Oksana Shynlova7. Identification of Connexin43 Phosphorylation and S -Nitrosylation in Cultured Primary Vascular CellsAlexander W. Lohman, Adam C. Straub, and Scott R. Johnstone8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome AnalysisStephanie Fowler, Mark Akins, and Steffany A.L. Bennett9. Scrape Loading/Dye Transfer AssayPavel Babica, Iva Sovadinov , and Brad L. Upham10. Microinjection Technique for Assessment of Gap Junction FunctionMichael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional CouplingElke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In VitroMaria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging Song Yang and Wen-Hong Li14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone CellsJoseph P. Stains and Roberto Civitelli15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels", "item_img_path" : "https://covers2.booksamillion.com/covers/bam/1/49/393/662/149393662X_b.jpg", "price_data" : { "retail_price" : "109.99", "online_price" : "109.99", "our_price" : "109.99", "club_price" : "109.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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