{ "item_title" : "Microcephaly", "item_author" : [" Timothy Gershon "], "item_description" : "Part I Cellular Analyses of Neural Progenitors1. Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular BiologyJennifer Karin Ocasio2. Maintaining Cerebellar Granule Neuron Progenitors in Cell CultureJennifer Karin Ocasio3. Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow CytometryJennifer Karin Ocasio4. Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron ProgenitorsGouri NanjangudPart II Analyses of Neural Progenitors In Situ5. Processing of Neonatal Mouse Brains for Immunohistochemical Analysis, As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and ApoptosisGabriela De la Cruz, Nana Nikolaishvili-Feinberg, Scott E. Williams, and Timothy R. Gershon6. Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain SectionsGabriela De la Cruz, Nana Nikolaishvili-Feinberg, and Timothy R. Gershon7. Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain SectionsGabriela De la Cruz, Nana Nikolaishvili-Feinberg, and Scott E. WilliamsPart III RNA-seq Analysis with Single-Cell Resolution (scRNA-seq)8. scRNA-seq for Microcephaly Research 1]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA SynthesisBenjamin Babcock and Seth Weir9. scRNA-seq for Microcephaly Research 2]: Preparation of Single-Cell cDNA LibrariesBenjamin Babcock and Seth Weir10. scRNA-seq for Microcephaly Research 3]: Computational Analysis of scRNA-seq DataBenjamin Babcock and Daniel Malawsky11. scRNA-seq for Microcephaly Research 4]: Dirichlet Regression for Single Cell Population DifferencesDaniel Malawsky and Timothy R. GershonPart IV MRI Analyses of the Mouse Brain12. Mouse Brain MRI, Including In Vivo, Ex Vivo, and fcMRI for the Study of MicrocephalyMartin J. MacKinnon, Tzu-Wen W. Wang, and Yen-Yu I. Shih13. Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly, using 13C IsotopesAndrey P. TikunovPart V Behavioral Phenotyping in Neonatal Mice14. Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental DisordersKathryn M. Harper, Viktoriya D. Nikolova, Monika E. Conrad, and Sheryl S. Moy", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/1/07/162/751/1071627511_b.jpg", "price_data" : { "retail_price" : "219.99", "online_price" : "219.99", "our_price" : "219.99", "club_price" : "219.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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