{ "item_title" : "Microinjection", "item_author" : [" Chengyu Liu", "Yubin Du "], "item_description" : "1. Microinjection and Micromanipulation: A Historical PerspectiveWenhao Xu2. Production of Transgenic Mice by Pronuclear MicroinjectionXin-an Pu, Anthony P. Young, and H. Michael Kubisch3. Transgene Recombineering in Bacterial Artificial ChromosomesMichael G. Zeidler and Thomas L. Saunders4. Using TARGATT TM Technology to Generate Site-Specific Transgenic MiceRuby Yanru Chen-Tsai5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated MethodTakuya Sato and Takehiko Ogawa6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine TransferYubin Du, Wen Xie, Fan Zhang, and Chengyu Liu7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug SelectionPinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG MiceYubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection TechniqueMelissa A. Scott and Yueh-Chiang Hu10. Delivery of CRISPR-Cas9 into Mouse Zygotes by ElectroporationWenning Qin and Haoyi Wang11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA OligonucleotidesYe Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear InjectionSayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and ApplicationsNaomi Nakagata, Jorge Sztein, and Toru Takeo14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed SpermToru Takeo, Jorge Sztein, and Naomi Nakagata15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse LineTakayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 TechnologyTuija M. Alcantar and Xin Y. Rairdan17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos &nbs", "item_img_path" : "https://covers4.booksamillion.com/covers/bam/1/49/398/830/1493988301_b.jpg", "price_data" : { "retail_price" : "249.99", "online_price" : "249.99", "our_price" : "249.99", "club_price" : "249.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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