{ "item_title" : "Multiphoton Microscopy and Fluorescence Lifetime Imaging", "item_author" : [" Karsten König", "Thérèse Baldeweck", "Mihaela Balu "], "item_description" : "This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard.Contents Part I: BasicsBrief history of fluorescence lifetime imagingThe long journey to the laser and its use for nonlinear opticsAdvanced TCSPC-FLIM techniquesUltrafast lasers in biophotonicsPart II: Modern nonlinear microscopy of live cellsSTED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensitiesPrinciples and applications of temporal-focusing wide-field two-photon microscopyFLIM-FRET microscopyTCSPC FLIM and PLIM for metabolic imaging and oxygen sensingLaser tweezers are sources of two-photon effectsMetabolic shifts in cell proliferation and differentiationFemtosecond laser nanoprocessingCryomultiphoton imagingPart III: Nonlinear tissue imagingMultiphoton Tomography (MPT)Clinical multimodal CARS imagingIn vivo multiphoton microscopy of human skinTwo-photon microscopy and fluorescence lifetime imaging of the corneaMultiscale correlative imaging of the brainRevealing interaction of dyes and nanomaterials by multiphoton imagingMultiphoton FLIM in cosmetic clinical researchMultiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgeryNon-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse modelsBedside assessment of multiphoton tomography", "item_img_path" : "https://covers4.booksamillion.com/covers/bam/3/11/043/898/3110438984_b.jpg", "price_data" : { "retail_price" : "194.99", "online_price" : "194.99", "our_price" : "194.99", "club_price" : "194.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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