Pharmacogenomics : Methods and Protocols
Overview
Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.
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Details
- ISBN-13: 9781617375712
- ISBN-10: 1617375713
- Publisher: Humana
- Publish Date: November 2010
- Dimensions: 9 x 6 x 0.48 inches
- Shipping Weight: 0.68 pounds
- Page Count: 214
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