Poly(adp-Ribose) Polymerase : Methods and Protocols
Overview
Part I: Detection and Quantification of pADPr 1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry Tabea Zubel, Rita Martello, Alexander B rkle, and Aswin Mangerich 2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies Eszter M. Horvath, Zsuzsanna K. Zsengell r, and Csaba Szabo 3. Detecting and Quantifying pADPr In Vivo Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark 4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian D lle 5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry Jone Michelena and Matthias Altmeyer Part II: Identification of Protein Targets 6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe Rory K. Morgan and Michael S. Cohen 7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung 8. Using Clickable NAD + Analogues to Label Substrate Proteins of PARPs Lu Zhang and Hening Lin 9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a 'Clickable' NAD + Analog Bryan A. Gibson and W. Lee Kraus 10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography - Tandem Mass Spectrometry Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger 11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography -Tandem Mass Spectrometry Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen Part III: Functional Analysis 12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair Th o Lebeaupin, Rebecca Smith, S bastien Huet, and Gyula Timinszky 13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function Edit Mik , T nde Kov cs, Tam s Fodor, and P ter Bai 14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay Yingbiao Ji 15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)
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Details
- ISBN-13: 9781493969920
- ISBN-10: 1493969927
- Publisher: Humana
- Publish Date: July 2017
- Dimensions: 10 x 7 x 1.19 inches
- Shipping Weight: 2.56 pounds
- Page Count: 528
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