Protein Complex Assembly : Methods and Protocols
Overview
PART I - Experimental methods 1. Experimental Characterisation Of Protein Complex Structure, Dynamics, and Assembly Jonathan N. Wells and Joseph A. Marsh 2. High-Throughput Electron Cryo-Tomography of Protein Complexes and their Assembly Louie D. Henderson and Morgan Beeby 3. Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single Particle Electron Microscopy Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes, and Deborah F. Kelly 4. Time-resolved Cryo-Electron Microscopy Using a Microfluidic Chip Sandip Kaledhonkar, Ziao Fu, Howard White and Joachim Frank 5. Characterizing Protein-protein Interactions using Solution NMR Spectroscopy Jose Luis Ortega-Roldan, Martin Blackledge and Malene Ringkj bing Jensen 6. Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit For Solution-state and Solid-state NMR Emeline Barbet-Massin, Eli van der Sluis, Joanna Musial, Roland Beckmann, and Bernd Reif 7. Characterizing Protein-Protein Interactions using Deep Sequencing Coupled to Yeast Surface Display Angelica V. Medina-Cucurella and Timothy A. Whitehead 8. Structurally Guided In Vivo Crosslinking Johanna C. Scheinost and Thomas G. Gligoris 9. Characterising Intact Macromolecular Complexes using Native Mass Spectrometry Elisabetta Boeri Erba, Luca Signor, Mizar F. Oliva, Fabienne Hans, and Carlo Petosa 10. Hydrogen Deuterium Exchange Mass Spectrometry to Study Protein Complexes Brent A. Kochert, Roxana E. Iacob, Thomas E. Wales, Alexandros Makriyannis, and John R. Engen 11. Structural Analysis of Protein Complexes by Cross-linking and Mass-spectrometry Moriya Slavin and Nir Kalisman 12. Global Characterization of Protein Complexes by Biochemical Purification-mass Spectrometry (BP/MS) Reza Pourhaghighi and Andrew Emili 13. Proteomic Profiling of Integrin Adhesion Complex Assembly Adam Byron 14. Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies Philipp Hoess, Markus Mund, Manuel Reitberger and Jonas Ries 15. Correlative 3D Structured Illumination Microscopy and Single Molecular Localisation Microscopy for Imaging Cancer Invasion Shannon J.L. Pinnington, John F. Marshall and Ann P. Wheeler 16. Observing the Assembly of Protein Complexes In Living Eukaryotic Cells In Super-resolution using refSOFI Fabian Hertel, Gary C.H. Mo, Peter Dedecker and Jin Zhang 17. Detecting Purinosome Metabolon Formation with Fluorescence Microscopy Anthony M. Pedley and Stephen J. Benkovic 18. Analysis of Bacterial pilus Assembly by Shearing and Immunofluorescence Microscopy Areli Luna-Rico, Jenny-Lee Thomassin and Olivera Francetic 19. Expression, Purification and Assembly of Archaellum Subcomplexes of Sulfolobus Acidocaldarius Paushali Chaudhury, Patrick Tripp, and Sonja-Verena Albers 20. Unstable Protein Purification through the Formation of Stable Complexes Sylvia Eiler, Nicolas Levy, Benoit Maillot, Julien Batisse, Karine Pradeau Aubreton, Oyindamola Oladosu, and Marc Ruff 21. Expressing Multi-subunit Complexes using biGBac Florian Weissmann and Jan-Michael Peters PART II - Computational Methods 22. Computational Modelling of Protein Complex Structure and Assembly Jonathan N.
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Details
- ISBN-13: 9781493977581
- ISBN-10: 149397758X
- Publisher: Humana
- Publish Date: April 2018
- Dimensions: 10 x 7 x 1.13 inches
- Shipping Weight: 2.47 pounds
- Page Count: 506
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