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RNA Detection and Visualization|Jeffrey E. Gerst

RNA Detection and Visualization : Methods and Protocols

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Overview

Part I: Visualizing mRNAs In situ 1. Single Molecule Imaging of RNA In situ Mona Batish, Arjun Raj and Sanjay Tyagi 2. FISH and Immunofluorescence Staining in Chlamydomonas James Uniacke, Daniel Col n-Ramos, and William Zerges 3. High Resolution Fluorescent In situ Hybridization in Drosophila Eric L cuyer 4. Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization Alexandre Paix, Janet Chenevert, and Christian Sardet 5. Visualization of mRNA Localization in Xenopus Oocytes James A. Gagnon and Kimberly L. Mowry 6. Visualization of mRNA Expression in the Zebrafish Embryo Yossy Machluf and Gil Levkowitz 7. High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo Sharon A. Swanger, Gary J. Bassell, and Christina Gross 8. Localization of mRNA in Vertebrate Axons Using In situ Hybridization Jos Roberto Sotelo-Silveira, Aldo Calliari, Alejandra Kun, Victoria Elizondo, Luc a Canclini, and Jos Roberto Sotelo Part II: Visualizing mRNAs In vivo Using Molecular Probes or Reconstituted Fluorescent Proteins 9. Tiny Molecular Beacons for In vivo mRNA Detection Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras 10. Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA Antony K. Chen, Won Jong Rhee, Gang Bao, and Andrew Tsourkas 11. Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells Takeaki Ozawa and Yoshio Umezawa 12. Visualization of Induced RNA in Single Bacterial Cells Azra Borogovac and Natalia E. Broude Part III: Visualizing mRNAs In vivo Using Aptamers and Intact Fluorescent Proteins 13. Visualizing mRNAs in Fixed and Living Yeast Cells Franck Gallardo and Pascal Chartrand 14. In vivo Visualization of RNA Using the U1A-Based Tagged RNA System Sunglan Chung and Peter A. Takizawa 15. Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy Liora Haim-Vilmovsky and Jeffrey. E. Gerst 16. Imaging mRNAs in Living Mammalian Cells Sharon Yunger and Yaron Shav-Tal 17. Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis Katsiaryna Belaya and Daniel St. Johnston Part IV: Use of Cell Fractionation to Demonstrate the Sub-Cellular L ocalization of RNA 18. Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria Erez Eliyahu, Daniel Melamed, and Yoav Arava 19. Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation Sujatha Jagannathan, Christine Nwosu, and Christopher V. Nicchitta 20. Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure Gadi Zipor, Cecile Brocard, and Jeffrey. E. Gerst 21. Profiling Axonal mRNA Transport

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Details

  • ISBN-13: 9781493958368
  • ISBN-10: 1493958364
  • Publisher: Humana
  • Publish Date: August 2016
  • Dimensions: 10 x 7 x 1.03 inches
  • Shipping Weight: 1.94 pounds
  • Page Count: 497

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