Overview
Part I: Bioinformatic Tools to Study RNA Modifications 1. RNA Post-Transcriptional Modification Mapping Data Analysis Using RNA Framework Ilaria Manfredonia and Danny Incarnato 2. An Informatics Pipeline for Profiling and Annotating RNA Modifications Qi Liu, Xiaoqiang Lang, and Richard I. Gregory Part II: Detecting RNA Modifications Using Nanopore Direct RNA Sequencing 3. EpiNano : Detection of m 6 A RNA Modifications Using Oxford Nanopore Direct RNA Sequencing Huanle Liu, Oguzhan Begik, and Eva Maria Novoa 4. Adaptation of Human Ribosomal RNA for Nanopore Sequencing of Canonical and Modified Nucleotides Miten Jain, Hugh E. Olsen, Mark Akeson, and Robin Abu-Shumays Part III: Next-Generation Sequencing Approaches to Detect and Capture Modified RNAs 5. AlkAniline-Seq: A Highly Sensitive and Specific Method for Simultaneous Mapping of 7-Methyl-Guanosine (m 7 G) and 3-Methyl-Cytidine (m 3 C) in RNAs by High-Throughput Sequencing Virginie Marchand, Lilia Ayadi, Val rie Bourguignon-Igel, Mark Helm, and Yuri Motorin 6. Transcriptome-Wide Detection of Internal N 7 -Methylguanosine Li-Sheng Zhang, Chang Liu, and Chuan He 7. miCLIP-MaPseq Identifies Substrates of Radical SAM RNA Methylating Enzyme Using Mechanistic Crosslinking and Mismatch Profiling Vanja Stojkovic, David E. Weinberg, and Danica Galonic Fujimori 8. Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing Bryan R. Cullen and Kevin Tsai 9. Quantitative and Single Nucleotide Resolution Profiling of RNA 5-Methylcytosine Jun Li, Xingyu Wu, Trung Do, Vy Nguyen, Jing Zhao, Pei Qin Ng, Alice Burgess, Rakesh David, and Iain Searle 10. A Small RNA-Seq Protocol with Less Bias and Improved Capture of 2'-O-Methyl RNAs Erwin L. van Dijk and Claude Thermes Part IV: Assessing RNA Modifications Using qPCR- and Molecular Biology-Based Methods 11. Assessing 2'- O -Methylation of mRNA Using Quantitative PCR Brittany A. Elliott and Christopher L. Holley 12. Relative Quantification of Residue Specific m6A RNA Methylation Using m6A-RT-QPCR Ane Olazagoitia-Garmendia and Ainara Castellanos-Rubio 13. Monitoring the 5-Methoxycarbonylmethyl-2-Thiouridine (mcm5s2U) Modification Utilizing the Gamma-Toxin Endonuclease Jenna M. Lentini and Dragony Fu 14. Analysis of Queuosine tRNA Modifications Using APB Northern Blot Assays Cansu Cirzi and
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Details
- ISBN-13: 9781071613733
- ISBN-10: 1071613731
- Publisher: Humana
- Publish Date: June 2021
- Dimensions: 10.2 x 7.7 x 1 inches
- Shipping Weight: 2.1 pounds
- Page Count: 427
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