{ "item_title" : "Recombinant and in Vitro RNA Synthesis", "item_author" : [" Graeme L. Conn "], "item_description" : "1. Purification of RNA Expressed in vivo Inserted in a tRNA ScaffoldLuc Ponchon and Fr d ric Dardel2. Selective RNase H Cleavage of Target RNAs from a tRNA ScaffoldLuc Ponchon, Genevi ve Beauvais, Sylvie Nonin-Lecomte and Fr d ric Dardel3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCRJessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGEJo L. Linpinsel and Graeme L. Conn5. Preparation of Short RNA by in vitro TranscriptionCheng Lu and Pingwei Li6. Native RNA Purification by Gel Filtration ChromatographyEvan P. Booy, Hui Meng and Sean A. McKenna7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' EndsJohanna M. Avis, Graeme L. Conn, and Scott C. Walker8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' EndsMilena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme MethodsHae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNAGenevi ve Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault11. Affinity Purification of RNA using an ARiBo TagGenevi ve Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompr -Brodeur, Vanessa Delfosse and Pascale Legault12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing ExperimentsVirginia K. Vachon and Graeme L. Conn13. In Vitro Transcription of Modified RNAsStephanie L. Moon and Jeffrey Wilusz14. End-Labeling Oligonucleotides with Chemical Tags After SynthesisN. Ruth Zearfoss and Sean P. Ryder15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNAYa-Ming Hou16. Se-derivatized RNAs for X-ray CrystallographyLina Lin and Zhen Huang17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAsLuigi Martino and Maria R Conte18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLCPierre Dagenais and Pascale Legault19. Splint Ligation of RNA with T4 DNA LigaseChristopher J. Kershaw and Raymond T. O'Keefe", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/1/49/395/830/1493958305_b.jpg", "price_data" : { "retail_price" : "139.00", "online_price" : "139.00", "our_price" : "139.00", "club_price" : "139.00", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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