{ "item_title" : "Structural Genomics", "item_author" : [" Yu Wai Chen "], "item_description" : "Part I. Cloning, Expression and Protein Production 1. DisMeta - a Meta Server for Construct Design and OptimizationYuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione2. Stable Expression Clones and Auto-Induction for Protein Production in E. Coli F. William Studier 3. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli Natalie J. Saez and Renaud Vincentelli 4. Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning Claire Strain-Damerell, Nicola A. Burgess-Brown, Pravin Mahajan, and Opher Gileadi 5. Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, and Susanne Gr slund 6. Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells Pravin Mahajan, Nicola A. Burgess-Brown, Claire Strain-Damerell, and Opher Gileadi 7. OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger 8. Multiprotein Complex Production in Insect Cells by Using Polyproteins Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, and Imre Berger 9. Expression Screening in Mammalian Suspension CellsSusan D. Chapple and Michael R. Dyson 10. Cell-free Expression of Protein Complexes for Structural Biology Takaho Terada, Takeshi Murata, Mikako Shirouzu, and Shigeyuki Yokoyama 11. Cell-Free Protein Synthesis for Functional and Structural Studies Shin-ichi Makino, Emily T. Beebe, John L. Markley, and Brian G. Fox 12. Insoluble Protein Purification with Sarkosyl: Facts and Precautions Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, and Yu Wai Chen Part II. Experimental Structure Determination and Characterisation 13. Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay Vincent Mariaule, Florine Dupeus, and JosA. M rquez 14. CrystalDirect(TM) A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin FilmsJose A. Marquez and Florent Cipriani 15. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction AnisotropyMichael R. Sawaya16. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure DeterminationYunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy 17. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet, and Jerome Boisbouvier 18. High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, and Greg L. Hura 19. Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass SpectrometryFranz Herzog Part III. Computational Methods and Structural Data Analyses 20. Modeling of Proteins and Their Assemblies with the Integrative Modeling PlatformBenjamin Webb, Ker", "item_img_path" : "https://covers1.booksamillion.com/covers/bam/1/62/703/690/1627036903_b.jpg", "price_data" : { "retail_price" : "109.99", "online_price" : "109.99", "our_price" : "109.99", "club_price" : "109.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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