{ "item_title" : "In Vitro Guide", "item_author" : [" Kevin Thomsone "], "item_description" : "In Vitro Guide: Tissue Culture Protocols for Aquarium Plants - 50 Aquatic Plant Protocols for Successful PropagationUnlock the science of in vitro plant culture and take your aquascaping or aquatic plant business to the next level. In Vitro Guide: Tissue Culture Protocols for Aquarium Plants is the ultimate hands-on reference for anyone serious about growing aquatic plants through sterile, laboratory-grade techniques. Designed for hobbyists, commercial growers, and aquascaping professionals, this book delivers 50 complete, step-by-step protocols-each tested and refined for real-world application, giving you reliable results every time.Inside you'll find detailed instructions on explant preparation, sterilization techniques, media formulations, hormone concentrations, shoot induction, rooting, multiplication cycles, and acclimatization. Every protocol is built around practical lab workflows that scale from small DIY setups to professional production. Whether you're producing rare aquascaping species for personal collections or planning commercial micropropagation, this guide is designed to save you years of trial and error.The book covers a wide range of aquatic plants, including iconic stem plants, carpeting groundcovers, grasses, rosette plants, swords, rhizomatous species, mosses, and crypts. Each species has its own optimized recipe so you can maximize survival rates, multiplication, and transition success from sterile agar to thriving submersed growth.Plants included in this book: Rotala rotundifolia, Rotala wallichii, Rotala macrandra, Rotala sp. Colorata, Ludwigia repens, Ludwigia arcuata, Ludwigia glandulosa, Ludwigia palustris Super Red, Bacopa caroliniana, Bacopa monnieri, Limnophila sessiliflora, Limnophila aromatica, Hygrophila polysperma, Hygrophila corymbosa, Hygrophila pinnatifida, Micranthemum tweediei Monte Carlo, Micranthemum umbrosum, Hemianthus callitrichoides Cuba, Hemianthus micranthemoides, Glossostigma elatinoides, Hydrocotyle tripartita Japan, Lilaeopsis brasiliensis, Lilaeopsis mauritiana, Marsilea hirsuta, Marsilea crenata, Eleocharis acicularis dwarf hairgrass, Eleocharis parvula mini hairgrass, Sagittaria subulata, Helanthium tenellum Echinodorus tenellus, Hydrocleys nymphoides, Juncus repens, Cryptocoryne wendtii green brown red, Cryptocoryne lutea, Cryptocoryne beckettii Petchii, Cryptocoryne pontederiifolia, Cryptocoryne albida, Cryptocoryne crispatula Balansae, Echinodorus Reni, Echinodorus bleheri, Barclaya longifolia, Staurogyne repens, Pogostemon helferi, Pogostemon erectus, Bolbitis heudelotii, Anubias barteri Nana, Anubias barteri Petite, Bucephalandra Wavy Green, Bucephalandra Red Mini, Taxiphyllum barbieri Java Moss.By including the most sought-after aquarium plants in the hobby-such as Rotala, Ludwigia, Cryptocoryne, Anubias, Pogostemon, Bucephalandra, and more-this book ensures you'll always have access to professional-grade recipes. Each chapter is clearly structured, so even if you're a beginner with no prior experience in plant tissue culture, you can follow along and succeed.This guide is not just about science-it's about building independence as an aquarist, eliminating pests and algae, and ensuring disease-free, vigorous plants that thrive in aquariums. For professionals, it opens the door to consistent production, scalability, and profit. For hobbyists, it's a chance to unlock rare species and maintain a steady supply of healthy, beautiful plants for personal tanks.If you are ready to transform your understanding of aquatic plants and gain the ability to propagate them cleanly, reliably, and at scale, this comprehensive book is your essential lab companion.", "item_img_path" : "https://covers2.booksamillion.com/covers/bam/9/79/826/827/9798268275445_b.jpg", "price_data" : { "retail_price" : "29.99", "online_price" : "29.99", "our_price" : "29.99", "club_price" : "29.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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