{ "item_title" : "Crispr Guide RNA Design", "item_author" : [" Tudor A. Fulga", "David J. H. F. Knapp", "Quentin R. V. Ferry "], "item_description" : "Part I: In Silico Design and Optimization of Guide RNA Sequences1. Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR ExperimentsFlorian Heigwer and Michael Boutros2. Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-AnalyzerGue-Ho Hwang, Jin-Soo Kim, and Sangsu BaePart II: Chemically-Modified Guide RNAs3. Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor CellsJenny Shapiro, Adi Tovin, Ortal Iancu, Daniel Allen, and Ayal Hendel4. Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNAMoira A. McMahon and Meghdad Rahdar5. 'Split-and-Click' sgRNALapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, and Tom Brown6. Chimeric DNA-RNA Guide RNA DesignsShuhan Lu, Ying Zhang, and Hao YinPart III: Expanding the CRISPR Toolbox7. Harnessing tRNA for Processing Ability and Promoter ActivityDavid J.H.F. Knapp and Tudor A. Fulga8. Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA-ncRNA Expression ConstructsDavid M. Shechner9. Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAsQuentin R.V. Ferry10. Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12aHa Rim Shin, Jiyeon Kweon, and Yongsub KimPart IV: Characterization of CRISPR Efficacy and Specificity11. Methods for Measuring CRISPR/Cas9 DNA Cleavage in CellsChristopher R. Cromwell, Juan Jovel, and Basil P. Hubbard12. In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 SystemsChristopher R. Cromwell and Basil P. Hubbard13. Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-SeqDaesik Kim and Jin-Soo Kim14. Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral VectorXiaoling Wang, Youjun Wu, and Jiing-Kuan Yee15. Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS MethodRoberto Ballarino, Britta A.M. Bouwman, and Nicola Crosetto", "item_img_path" : "https://covers2.booksamillion.com/covers/bam/1/07/160/686/1071606867_b.jpg", "price_data" : { "retail_price" : "249.99", "online_price" : "249.99", "our_price" : "249.99", "club_price" : "249.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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