The Integrated Stress Response : Methods and Protocols
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Overview
Part I: Analysis of mRNA Translation Regulation 1. An Overview of Methods for Detecting eIF2 α Phosphorylation and the Integrated Stress Response Agnieszka Krzyzosiak, Aleksandra Pitera, and Anne Bertolotti 2. CRISPR-Based Screening for Stress Response Factors in Mammalian Cells Xiaoyan Guo and Martin Kampmann 3. Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq Gemma E. May and C. Joel McManus 4. Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response Alyssa M. English and Stephanie L. Moon 5. Quantitative Translation Proteomics Using mePROD Kevin Klann and Christian M nch 6. Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2 Martin D. Jennings and Graham D. Pavitt 7. Mammalian In Vitro Translation Systems Yulia Gonskikh, Valentina Pecoraro, and Norbert Polacek 8. Measuring Repeat-Associated Non-AUG (RAN) Translation Shaopeng Wang and Shuying Sun 9. Analysis of Ribosome Profiling Data Carine Legrand, Khanh Dao Duc, and Francesca Tuorto 10. Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling Michael J. Holmes, Jagannath Misra, and Ronald C. Wek 11. High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation Mohammad Shafieinouri, Britnie Santiago Membreno, and Colin Chih-Chien Wu 12. Fluorescence Intensity-Based eIF2B's Guanine Nucleotide-Exchange Factor Activity Assay Yusuke Sekine, David Ron, and Alisa F. Zyryanova Part II: Analysis of Interaction Networks and RNP Granules 13. Collective Learnings of Studies of Stress Granule Assembly and Composition Hadjara Sidib and Christine Vande Velde 14. Detecting Stress Granules in Drosophila Neurons Fabienne De Graeve, Nadia Formicola, Kavya Vinayan Pushpalatha, Akira Nakamura, Eric Debreuve, Xavier Descombes, and Florence Besse 15. Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation Anna-Carina S hnel, Nataliya I. Trushina, and Roland Brandt 16. Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics Xiaojing Sui,
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Details
- ISBN-13: 9781071619742
- ISBN-10: 1071619748
- Publisher: Humana
- Publish Date: February 2022
- Dimensions: 10.32 x 7.3 x 0.9 inches
- Shipping Weight: 1.7 pounds
- Page Count: 404
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