{ "item_title" : "Retinal Development", "item_author" : [" Shu-Zhen Wang "], "item_description" : "Part I: Manipulation of Gene Expression in vivo 1. Conditional Control of Gene Expression in the Mouse Retina Qian Ding and Lin Gan 2. Generation Transgenic Xenopus using Restriction Enzyme-Mediated Intergration Mohammad Haeri and Barry E. Knox 3. In vivo Functional Analysis of Transcription Factor--response Element Interaction using Transgenic Xenopus laevis Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly 4. Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Johnson, Lennart Berglin, T. Michael Redmond, Jeffrey H. Boatright 5. In Ovo Eye Electroporation Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari 6. Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos Sally A. Moody Also see Chapter 8.Part II: Tracing Cell Fate 7. Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation and Explant Culture Sally A. Moody 8. Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman 9. Fate Tracing of neurogenin2 -expressing Cells in the Mouse Retina using CreER TM - LacZ Wenxin Ma and Shu-Zhen Wang Part III: In vitro Systems 10. In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development Kangxin Jin and Mengqing Xiang 11. In vitro Biochemical Assays to Monitor Rhodopsin Function Joshua Sammons and Alecia K. Gross 12. Transfection of Primary Embryonic Chicken Retinal Cells using Cationic Lipid Yi-Wen Hsieh and Xian-Jie Yang 13. Production of High Titer RCAS Retrovirus Run-Tao Yan and Shu-Zhen Wang 14. Chick RPE Transdifferentiation Assay for Proneural Activities Shu-Zhen Wang and Run-Tao Yan Part IV: Regeneration/Stem Cells 15. Studying the Generation of Regenerated Retinal Neuron from M ller glia in the Mouse Eye Mike O. Karl and Thomas A. Reh 16. Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells Anna La Torre, Deepak A Lamba, Anu Jayabalu, Thomas A Reh 17. Light-induced Photoreceptor Degeneration in the Retina of the Zebrafish Scott Taylor, Jing Chen, Jing Luo and Peter Hitchcock 18. Microarray-based Gene Profiling Analysis of M ller Glia-derived Retinal Stem Cells in Light-damaged Retinas from Adult Zebrafish Zhao Qin and Pamela A. Raymond Also see Chapter 8. Part V: Function/Imaging 19: Measuring Rodent Electroretinograms to Assess Retinal Function Molly E. Clark and Timothy W. Kraft 20. Functional Imaging of Retinal Photoreceptors and Inner Neurons using Stimulus-evoked Intrinsic Optical Signals Xin-Cheng Yao and Yi-Chao Li Part VI: Emerging Methodologies 21. Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs,and Donald J.Zack 22. Revealing Looping Organization of Mammalian Photoreceptor Genes Using Chromosome Conformation Capture (3C) Assays Guang-Hua Peng and Shiming Chen 23. Retinal Transcriptome Profiling by Directional Next Generation Sequencing Using 100 ng of Total RNA Matthew John Brooks, Harsha Karur Rajasimha, and Anand Swaroop 24. Exome Sequencing: Capture and Sequencing of All Human Coding Regions for Disease Gene Discovery Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew Brooks, and Anand Swaroop 25. Determinati", "item_img_path" : "https://covers3.booksamillion.com/covers/bam/1/61/779/847/1617798479_b.jpg", "price_data" : { "retail_price" : "109.99", "online_price" : "109.99", "our_price" : "109.99", "club_price" : "109.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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