{ "item_title" : "Germline Development", "item_author" : [" Wai-Yee Chan", "Le Ann Blomberg "], "item_description" : "Part I: Male Germline1. Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular StudiesYunmin Li, Teruko Taketo, and Yun-Fai Chris Lau2. Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical AnalysisGurpreet Manku, Monty Mazer, and Martine Culty3. Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter MiceThomas Garcia and Marie-Claude Hofmann4. Isolation of Human Male Germline Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell SortingZuping He, Maria Kokkinaki, Jiji Jiang, Wenxian Zeng, Ina Dobrinski, and Martin Dym5. Isolation and Purification of Murine Male Germ CellsCatherine Boucheron and Vanessa Baxendale6. Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell SuspensionsYan Wang and Martine Culty7. Revealing Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling MicroarrayTin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan8. Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha AcetyltransferaseAlan Lap-Yin Pang9. Identification of Novel Long Non-Coding RNA Transcripts in Male Germ CellsTin-Lap Lee, Amy Xiao, and Owen M. Rennert10. Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array HybridizationHoi-Hung Cheung, Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan11. Spermatogenesis in CryptorchidismAlexander I. Agoulnik, Zaohua Huang, and Lydia FergusonPart II: Female Germline12. In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular DevelopmentShyamal K. Roy, Cheng Wang, Anindit Mukherjee, and Prabuddha Chakraborty13. Microspread Oocyte Preparations for the Analysis of Meiotic Prophase Progression with Improved Recovery by Cytospin CentrifugationTeruko Taketo14. In Vitro Maturation (IVM) of Porcine OocytesYe Yuan and Rebecca L. Krisher15. Experimental Approaches to the Study of Human Primordial Germ CellsAndrew J. Childs and Richard A. Anderson16. Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and ImmunohistochemistryChang Liu, Melissa Paczkowski, Manal Othman, and Humphrey Hung-Chang Yao17. DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing MethodsHisato Kobayashi and Tomohiro Kono18. Gene Expression in Mouse Oocytes by RNA-SeqEric Antoniou and Robert Taft", "item_img_path" : "https://covers2.booksamillion.com/covers/bam/1/61/779/435/161779435X_b.jpg", "price_data" : { "retail_price" : "109.99", "online_price" : "109.99", "our_price" : "109.99", "club_price" : "109.99", "savings_pct" : "0", "savings_amt" : "0.00", "club_savings_pct" : "0", "club_savings_amt" : "0.00", "discount_pct" : "10", "store_price" : "" } }

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